Sosa Camila, Tirante Liliana, Chaves Javier, Pol Martín, Ambrogi Arnaldo, Giraudo José Angel, Tamiozzo Pablo
Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, República Argentina.
Laboratorio Lactodiagnóstico Sur Sociedad Responsabilidad Limitada (SRL), Olivos, Buenos Aires, República Argentina.
Rev Argent Microbiol. 2018 Jan-Mar;50(1):31-35. doi: 10.1016/j.ram.2017.02.010. Epub 2017 Sep 28.
Several species of Mycoplasma and Ureaplasma diversum can cause diseases in dairy cattle, which can be associated or not with clinical manifestations. In our country, the presence of Mycoplasma bovis, Mycoplasma californicum and Mycoplasma canadense has been detected, being the only mycoplasma species identified so far. The objective of this study was to identify other species of the Mycoplasmataceae family. Thirty-five Mycoplasma spp.-like isolates obtained from different samples from cattle, with or without clinical symptoms, from eight herds located in the provinces of Santa Fe, Cordoba, Buenos Aires and San Luis were utilized in the present study. Through the use of species-specific polymerase chain reactions (PCR) Mycoplasma bovigenitalium, Mycoplasma alkalescens, Mycoplasma bovirhinis and U. diversum were identified and through amplification and further sequencing of the 16-23S rRNA intergenic spacer regions, Mycoplasma arginine and M. californicum were identified. The identification of these species represents an important advance in knowledge in order to include these pathogens in the differential diagnosis of certain clinical and pathological entities of cattle from Argentina.
几种支原体和多样性脲原体可导致奶牛发病，这些疾病可能伴有或不伴有临床表现。在我国，已检测到牛支原体、加利福尼亚支原体和加拿大支原体的存在，它们是目前为止唯一已鉴定出的支原体物种。本研究的目的是鉴定支原体科的其他物种。本研究使用了从位于圣菲省、科尔多瓦省、布宜诺斯艾利斯省和圣路易斯省的8个牛群中采集的35株类似支原体属的分离株，这些分离株来自有或无临床症状的牛的不同样本。通过使用种特异性聚合酶链反应（PCR）鉴定出了牛生殖支原体、碱性支原体、牛鼻支原体和多样性脲原体，通过对16-23S rRNA基因间隔区进行扩增和进一步测序，鉴定出了精氨酸支原体和加利福尼亚支原体。这些物种的鉴定代表了知识上的一项重要进展，以便将这些病原体纳入阿根廷牛某些临床和病理实体的鉴别诊断中。
