Instituto de Investigación de la Cadena Láctea (INTA-CONCET), Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Rafaela, Santa Fe, Argentina.
Instituto de Investigación de la Cadena Láctea (INTA-CONCET), Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Rafaela, Santa Fe, Argentina; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina.
Rev Argent Microbiol. 2022 Apr-Jun;54(2):158-161. doi: 10.1016/j.ram.2021.07.001. Epub 2021 Sep 4.
There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M.canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milk and biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M.californicum and M.leachii were detected by using two multiplex PCRs, confirming their presence in clinical conditions in dairy cattle. The techniques used in the present study can be useful to broaden the knowledge about Mycoplasma infections in cattle, since the search for these organisms is not usually included in routine diagnoses.
关于阿根廷奶牛场中支原体属的存在频率以及与支原体属相关的流行病学和临床特征的信息很少。本研究的目的是开发一种用于鉴定 M.bovis 和 M.canadense 的多重 PCR，并描述从提交给诊断实验室的临床样本中分离出的支原体属的频率。在总共 1548 份来自乳房内感染、奶罐奶和生物液体的样本中，获得了 38 株支原体分离株。使用两种多重 PCR 检测到 M. bovis、M. canadense、M.californicum 和 M.leachii，证实它们在奶牛的临床条件下存在。本研究中使用的技术可以有助于扩大对牛支原体感染的认识，因为这些生物体的检测通常不包括在常规诊断中。
