通过酶联免疫吸附测定法检测因子XIIIa和转谷氨酰胺酶的转酰胺活性。
Transamidating activities of factor XIIIa and of transglutaminases, measured by an ELISA procedure.
作者信息
Velasco P T, Karush F, Lorand L
机构信息
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
出版信息
Biochem Biophys Res Commun. 1988 Apr 29;152(2):505-11. doi: 10.1016/s0006-291x(88)80066-4.
PMID:2896499
Abstract
The dansyl hapten in dansylcadaverine offers unique possibilities for measuring the incorporation of the monoamine into proteins (e.g. N,N'-dimethylcasein) by transamidating enzymes such as factor XIIIa and the transglutaminases. The protein-bound dansylcadaverine was assayed by an ELISA procedure based on a monoclonal antibody to the dansyl moiety.
摘要
丹磺酰尸胺中的丹磺酰半抗原为通过诸如因子XIIIa和转谷氨酰胺酶等转酰胺酶来测量单胺掺入蛋白质（如N,N'-二甲基酪蛋白）提供了独特的可能性。通过基于针对丹磺酰部分的单克隆抗体的ELISA程序来测定与蛋白质结合的丹磺酰尸胺。
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