A new thiourea derivative [2-(3-ethylthioureido)benzoic acid] for cloud point extraction of some trace metals in water, biological and food samples.

2-(3-Ethylthioureido)benzoic acid was prepared and characterized by electronic spectrum, elemental analysis, Fourier transform infrared spectroscopy, H nuclear magnetic resonance spectrum and mass spectrum. The produced ligand was applied for the preconcentrative of Fe, Co, Cu and Zn in aqueous samples by cloud point extraction methodology. Triton X-114 was used as extractant. Experimental parameters that may affect the extraction process were examined and optimized; such as pH, ligand and triton concentrations, type of diluting solvent, extraction temperature and ionic strength. The calibration curves were linear upto 500μgL for Fe, Cu and Zn and upto 200μgL for Co. The achieved detection limits were 1.5, 0.23, 0.71 and 0.35μgL for Fe, Co, Cu and Zn respectively. The accuracy was established by analysis of certified reference materials (Seronorm whole blood L2 and ZCS ZC85006 Tomato). The proposed procedure was used for preconcentration of these metal ions in water, biological and food samples prior to their determination by flame atomic absorption spectrometry.