Department of Chemical Sciences, Faculty of Pharmacy, Medical University of Plovdiv, 120 Buxton Bros Str., 4004 Plovdiv, Bulgaria.
Faculty of Pharmacy, İstanbul Yeni Yüzyıl Üniversitesi, 26 Yılanlı Ayazma Caddesi, 34010 İstanbul, Turkey.
Molecules. 2022 Jul 24;27(15):4725. doi: 10.3390/molecules27154725.
The hydrophobic azo dye 6-hexyl-4-(2-thiazolylazo)resorcinol (HTAR, HL) was studied as part of a system for the centrifuge-less cloud point extraction (CL-CPE) and spectrophotometric determination of traces of cobalt. The extracted 1:2 (Co:HTAR) complex, [Co(HL)(L)], shows an absorption maximum at 553 nm and contains HTAR in two different acid-base forms. Optimum conditions for its formation and CL-CPE were found as follows: 1 × 10 mol L of HTAR, 1.64% of Triton X-114, pH of 7.8, incubation time of 20 min at ca. 50 °C, and cooling time of 30 min at ca. -20 °C. The linear range, limit of detection, and apparent molar absorptivity coefficient were 5.4-189 ng mL, 1.64 ng mL, and 2.63 × 10 L mol cm, respectively. The developed procedure does not use any organic solvents and can be described as simple, cheap, sensitive, convenient, and environmentally friendly. It was successfully applied to the analysis of artificial mixtures and real samples, such as steel, dental alloy, rainwater, ampoules of vitamin B, and saline solution for intravenous infusion.
疏水性偶氮染料 6-己基-4-(2-噻唑偶氮)间苯二酚（HTAR，HL）被研究作为无离心相分离分光光度法（CL-CPE）和痕量钴的测定系统的一部分。所提取的 1:2（Co:HTAR）络合物[Co（HL）（L）]在 553nm 处具有最大吸收，并且包含 HTAR 的两种不同酸碱形式。发现其形成和 CL-CPE 的最佳条件如下：HTAR 的浓度为 1×10 mol L，Triton X-114 的浓度为 1.64%，pH 值为 7.8，在 50°C 左右孵育 20min，在约-20°C 下冷却 30min。线性范围、检测限和表观摩尔吸光系数分别为 5.4-189ng mL、1.64ng mL 和 2.63×10 L mol cm。所开发的程序不使用任何有机溶剂，可以描述为简单、廉价、灵敏、方便和环保。它成功地应用于人工混合物和真实样品的分析，如钢、牙科合金、雨水、维生素 B 安瓿和静脉输液生理盐水。
