Ho L T, Pu H F, Sheu W J, Wang W C, Wang P S
Department of Physiology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Chin J Physiol. 1987;30(2):45-53.
The effect of somatostatin on glucoseinduced secretion of insulin and gastric inhibitory polypeptide (GIP) in rats was examined. Anesthetized male rats were catheterized via the right carotid artery before gastric infusion of 2 ml of 40% glucose through a syringe pump over 3 min. Ten micrograms somatostatin dissolved in 0.5 ml saline was injected intraperitoneally 10 min before oral glucose administration. Rats receiving 0.5 ml saline were used as controls. Blood samples were collected at -10, 0, 10, 20, 30, 45, 60, and 90 min after glucose infusion. Concentrations of GIP and insulin in rat plasma samples were measured by radioimmunoassay. The level of plasma glucose was determined by YSI glucose analyzer. The results revealed that the rise of plasma insulin and GIP after the glucose load was abolished by a preinjection of somatostatin. It is concluded that somatostatin at 10 micrograms inhibits the effects of oral glucose in stimulating the secretion of GIP from the gut, and insulin from the pancreas in normal fasted rats.
研究了生长抑素对大鼠葡萄糖诱导的胰岛素和胃抑制多肽（GIP）分泌的影响。在通过注射泵于3分钟内给麻醉的雄性大鼠经胃注入2 ml 40%葡萄糖之前，经右颈动脉插入导管。在口服葡萄糖给药前10分钟，将溶解于0.5 ml盐水中的10微克生长抑素腹腔注射。接受0.5 ml盐水的大鼠用作对照。在葡萄糖输注后-10、0、10、20、30、45、60和90分钟采集血样。通过放射免疫测定法测量大鼠血浆样品中GIP和胰岛素的浓度。用YSI葡萄糖分析仪测定血浆葡萄糖水平。结果显示，预先注射生长抑素可消除葡萄糖负荷后血浆胰岛素和GIP的升高。得出结论，10微克的生长抑素可抑制正常禁食大鼠口服葡萄糖刺激肠道GIP分泌以及胰腺胰岛素分泌的作用。
