Gan Han Ming, Tan Mun Hua, Lee Yin Peng, Schultz Mark B, Horwitz Pierre, Burnham Quinton, Austin Christopher M
Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3220, Australia; Genomics Facility, Tropical and Medicine Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia; School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia.
Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3220, Australia; Genomics Facility, Tropical and Medicine Biology Platform, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia; School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Petaling Jaya, Selangor, Malaysia.
Mol Phylogenet Evol. 2018 Jan;118:88-98. doi: 10.1016/j.ympev.2017.09.022. Epub 2017 Sep 28.
To further understand the evolutionary history and mitogenomic features of Australia's highly distinctive freshwater crayfish fauna, we utilized a recently described rapid mitogenome sequencing pipeline to generate 24 new crayfish mitogenomes including a diversity of burrowing crayfish species and the first for Astacopsis gouldi, the world's largest freshwater invertebrate. Whole mitogenome-based phylogeny estimates using both Bayesian and Maximum Likelihood methods substantially strengthen existing hypotheses for systematic relationships among Australian freshwater crayfish with evidence of pervasive diversifying selection and accelerated mitochondrial substitution rate among the members of the clade representing strongly burrowing crayfish that may reflect selection pressures for increased energy requirement for adaptation to terrestrial environment and a burrowing lifestyle. Further, gene rearrangements are prevalent in the burrowing crayfish mitogenomes involving both tRNA and protein coding genes. In addition, duplicated control regions were observed in two closely related Engaeus species, together with evidence for concerted evolution. This study significantly adds to the understanding of Australian freshwater crayfish evolutionary relationships and suggests a link between mitogenome evolution and adaptation to terrestrial environments and a burrowing lifestyle in freshwater crayfish.
为了进一步了解澳大利亚极具特色的淡水小龙虾动物群的进化历史和线粒体基因组特征，我们利用最近描述的快速线粒体基因组测序流程，生成了24个新的小龙虾线粒体基因组，其中包括多种穴居小龙虾物种，以及世界上最大的淡水无脊椎动物——古尔德螯虾（Astacopsis gouldi）的首个线粒体基因组。使用贝叶斯方法和最大似然法基于完整线粒体基因组的系统发育估计，极大地强化了关于澳大利亚淡水小龙虾系统关系的现有假说，有证据表明在代表强穴居小龙虾的分支成员中存在普遍的多样化选择和加速的线粒体替代率，这可能反映了适应陆地环境和穴居生活方式对能量需求增加的选择压力。此外，基因重排现象在穴居小龙虾的线粒体基因组中很普遍，涉及tRNA和蛋白质编码基因。此外，在两个亲缘关系密切的恩氏螯虾（Engaeus）物种中观察到了重复的控制区，同时还有协同进化的证据。这项研究显著增进了我们对澳大利亚淡水小龙虾进化关系的理解，并表明线粒体基因组进化与淡水小龙虾对陆地环境的适应以及穴居生活方式之间存在联系。
