Huang Huiyan, Singh Komudi, Hart Anne C
Department of Neuroscience, Brown University, Providence, RI USA.
Current address: Laboratory of Mitochondrial Biology & Metabolism, National Institute of Health, Bethesda, MD USA.
Bio Protoc. 2017 Mar 20;7(6). doi: 10.21769/BioProtoc.2174.
C. elegans sleep during development is regulated by genes and cellular mechanisms that are conserved across the animal kingdom (Singh et al., 2014; Trojanowski & Raizen, 2016). developmental sleep is usually assessed during the transition to adulthood, a 2.6 h time interval called lethargus (Raizen , 2008; Singh , 2011). During lethargus, animals cycle between periods of immobility (sleep bouts) and periods of active locomotion (motion bouts). Sleep bouts resemble sleep in other species based on behavioral criteria, including cessation of feeding and locomotion, increased arousal threshold for response to sensory stimulation, rapid reversibility, and homeostatic response to sleep loss. Several assays have been developed to study sleep in (Belfer , 2013; Bringmann, 2011; Nelson , 2013; Raizen , 2008). Here, we contribute a detailed protocol for assessment of sleep during lethargus, which has been used successfully by many research groups, incorporating simple microfluidic chambers, a low cost camera with lighting system, and computational analysis based on image subtraction. We note that this system could be easily adapted to assess sleep in any small animal.
秀丽隐杆线虫发育过程中的睡眠受整个动物界保守的基因和细胞机制调控（辛格等人，2014年；特罗扬诺夫斯基和赖曾，2016年）。发育性睡眠通常在向成年期过渡期间进行评估，这是一个2.6小时的时间间隔，称为休眠期（赖曾，2008年；辛格，2011年）。在休眠期，动物在静止期（睡眠周期）和主动运动期（运动周期）之间循环。根据行为标准，睡眠周期类似于其他物种的睡眠，包括停止进食和运动、对感觉刺激反应的觉醒阈值增加、快速可逆性以及对睡眠剥夺的稳态反应。已经开发了几种方法来研究[此处缺失具体研究对象]的睡眠（贝尔弗，2013年；布林曼，2011年；纳尔逊，2013年；赖曾，2008年）。在这里，我们提供了一个详细的方案，用于评估休眠期的[此处缺失具体研究对象]睡眠，许多研究小组已成功使用该方案，它结合了简单的微流控室、带照明系统的低成本相机以及基于图像减法的计算分析。我们注意到，该系统可以很容易地进行调整，以评估任何小型动物的睡眠。
