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通过检测膜 TNF-α 和 CD154 鉴定出的对克氏锥虫有反应的 T 细胞在恰加斯病患者体内的存在。

T cells responding to Trypanosoma cruzi detected by membrane TNF-α and CD154 in chagasic patients.

机构信息

Grupo de Ciencias Básicas Médicas, Facultad de Medicina, Universidad de los Andes, Bogotá, Colombia.

Facultad de Medicina, Pontificia Universidad Javeriana and Hospital Universitario San Ignacio, Bogotá, Colombia.

出版信息

Immun Inflamm Dis. 2018 Mar;6(1):47-57. doi: 10.1002/iid3.197. Epub 2017 Oct 1.

DOI:10.1002/iid3.197
PMID:28967229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5818450/
Abstract

INTRODUCTION

Chagas disease is a parasitic infection whose pathogenesis is related to parasite persistence and a dysfunctional cellular immune response. Variability in cytokine secretion among chronic Trypanosoma cruzi-infected patients might preclude the identification of the pool of antigen specific T cells. The goal of this study was to determine the fraction of T cells responding to T. cruzi antigen measured by the expression of membrane TNF-α and CD154.

METHODS

A total of 21 chagasic patients, 11 healthy and 5 non-chagasic cardiomyopathy controls were analyzed. PBMCs were short-term cultured in the presence of anti-CD28, anti-CD49d, anti-TNF-α, and TACE (TNF-α converting enzyme) inhibitor either under T. cruzi-lysate or polyclonal stimuli. Cells were stained with anti-CD3, anti-CD4, anti-CD8, and anti-CD154, and analyzed with flow cytometry.

RESULTS

CD4+ and CD8+ T cells in chagasic patients displayed higher percentages of membrane-bound TNF-α+ and CD154+ compared with controls after T. cruzi-antigen stimulation. Both markers displayed a positive correlation in the T cell subpopulations analyzed. Symptomatic chagasic patients were differentiated from asymptomatic patients based on the expression of CD154 and membrane TNF-α in TCD4+ and TCD8+ compartments, respectively.

CONCLUSIONS

These results show that both markers could be useful for assessing the pool of antigen-specific T cells in chronic chagasic patients.

摘要

简介

恰加斯病是一种寄生虫感染，其发病机制与寄生虫的持续存在和细胞免疫反应功能障碍有关。慢性感染克氏锥虫的患者细胞因子分泌的可变性可能会阻止抗原特异性 T 细胞的鉴定。本研究的目的是通过测定 T 细胞对 T. cruzi 抗原的反应，即通过细胞膜 TNF-α和 CD154 的表达来确定。

方法

共分析了 21 例恰加斯病患者、11 例健康对照和 5 例非恰加斯病心肌病对照。在存在抗 CD28、抗 CD49d、抗 TNF-α和 TNF-α转化酶（TACE）抑制剂的情况下，将 PBMC 短期培养，无论是在 T. cruzi 裂解物还是多克隆刺激物的存在下。用抗 CD3、抗 CD4、抗 CD8 和抗 CD154 对细胞进行染色，并通过流式细胞术进行分析。

结果

与对照组相比，恰加斯病患者的 CD4+和 CD8+T 细胞在 T. cruzi 抗原刺激后，细胞膜结合的 TNF-α+和 CD154+的百分比更高。在分析的 T 细胞亚群中，这两种标志物均呈正相关。根据 TCD4+和 TCD8+细胞中 CD154 和膜 TNF-α的表达，可将有症状的恰加斯病患者与无症状患者区分开来。

结论

这些结果表明，这两种标志物都可用于评估慢性恰加斯病患者的抗原特异性 T 细胞库。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/0f8b9ef461f0/IID3-6-47-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/91d95bd977c2/IID3-6-47-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/30767c04e693/IID3-6-47-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/6f2c33cd9619/IID3-6-47-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/0f8b9ef461f0/IID3-6-47-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/91d95bd977c2/IID3-6-47-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/30767c04e693/IID3-6-47-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/6f2c33cd9619/IID3-6-47-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7243/5818450/0f8b9ef461f0/IID3-6-47-g005.jpg

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