Burleigh D E
Department of Pharmacology & Therapeutics, London Hospital Medical College, UK.
J Pharm Pharmacol. 1988 Jan;40(1):55-7. doi: 10.1111/j.2042-7158.1988.tb05151.x.
There is evidence to suggest an abnormality of the colonic myenteric plexus in severe chronic constipation. The present study investigates whether this abnormality involves functional changes in the cholinergic innervation of human colon. Human taenia coli muscle strips (taenia), previously incubated with [3H]choline to radiolabel neuronal stores of acetylcholine, were subjected to electrical field stimulation (1 Hz or 10 Hz, 1 ms, 480 pulses at 200 mA). The stimulation evoked release of tritiated material, shown previously to accurately represent neural [3H] acetylcholine release, was depressed in tissue from constipated compared with non-constipated patients. Evoked release of tritiated material was reduced by storage of the taenia at 4 degrees C or by increasing the frequency of stimulation, but increased by stimulation during incubation with [3H]choline. The results indicate that reduced activity of cholinergic nerves may occur within the bowel wall of colon removed for severe chronic constipation.
有证据表明，严重慢性便秘患者的结肠肌间神经丛存在异常。本研究旨在调查这种异常是否涉及人类结肠胆碱能神经支配的功能变化。将预先用[³H]胆碱孵育以放射性标记乙酰胆碱神经元储存的人结肠带肌条（结肠带）置于电场刺激（1Hz或10Hz，1ms，200mA下480个脉冲）。与非便秘患者相比，便秘患者组织中由刺激诱发的、先前已证明能准确代表神经[³H]乙酰胆碱释放的氚标记物质的释放受到抑制。结肠带在4℃储存或增加刺激频率会使诱发的氚标记物质释放减少，但在与[³H]胆碱孵育期间进行刺激则会增加。结果表明，在因严重慢性便秘而切除的结肠肠壁内，胆碱能神经的活性可能降低。
