Department of Gastrointestinal Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, P.R. China.
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou 515041, P.R. China.
Biomed Pharmacother. 2017 Nov;95:1868-1875. doi: 10.1016/j.biopha.2017.09.069. Epub 2017 Oct 6.
The purpose of the present study was to evaluate the effects of cordycepin (CA) on N-nitrosodiethylamine (NDEA)-induced hepatocellular carcinomas (HCC) and explore its potential mechanisms. Mice were randomly assigned to four groups: control group, NDEA group, NDEA+CA (20mg/kg) group, NDEA+CA (40mg/kg) group. The animal of each group were given NDEA (100ppm) in drinking water. One hour later, CA, which was dissolved in PBS, were intragastrically administered for continuous seven days. The results showed that CA reduced the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver and serum. CA also reduced the levels of interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α), methane dicarboxylic aldehyde (MDA), and stored the activity of superoxygen dehydrogenises (SOD) in serum. CA could obviously attenuate the hepatic pathological alteration. Furthermore, CA effectively inhibited the phosphorylations of phosphatidylinositol 3 kinase(PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR). In conclusion, our research suggested that CA exhibited protective effects on NDEA-induced hepatocellular carcinomas via the PI3K/Akt/mTOR pathway.
本研究旨在评估蛹虫草素 (CA) 对 N-亚硝基二乙胺 (NDEA) 诱导的肝癌 (HCC) 的影响，并探讨其潜在机制。将小鼠随机分为四组：对照组、NDEA 组、NDEA+CA(20mg/kg)组、NDEA+CA(40mg/kg)组。每组动物均给予含 NDEA(100ppm)的饮用水。1 小时后，将溶解在 PBS 中的 CA 灌胃给药，连续给药 7 天。结果表明，CA 降低了肝和血清中丙氨酸氨基转移酶 (ALT) 和天冬氨酸氨基转移酶 (AST) 的活性。CA 还降低了白细胞介素-6 (IL-6)、白细胞介素-1β、肿瘤坏死因子-α (TNF-α)、甲烷二羧酸醛 (MDA) 的水平，并储存了血清中超氧化物歧化酶 (SOD) 的活性。CA 能明显减轻肝组织病理改变。此外，CA 能有效抑制磷酸肌醇 3 激酶 (PI3K)、蛋白激酶 B (Akt)、哺乳动物雷帕霉素靶蛋白 (mTOR) 的磷酸化。综上所述，本研究表明 CA 通过 PI3K/Akt/mTOR 通路对 NDEA 诱导的肝癌具有保护作用。
