Biology Department and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal.
Biology Department and CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.
Ecotoxicol Environ Saf. 2018 Jan;147:905-912. doi: 10.1016/j.ecoenv.2017.09.060. Epub 2017 Sep 28.
Pharmaceuticals are micropollutants often present in wastewater treatment systems. In this study, the potential impact of such micropollutants on the bacterial population within aerobic granular sludge (AGS) bioreactor was investigated. The AGS bacterial community structure and composition were accessed combining DGGE fingerprinting and barcoded pyrosequencing analysis. Both revealed the existence of a dynamic bacterial community, independently of the pharmaceuticals presence. The AGS microbiome at both phylum and class levels varied over time and, after stopping pharmaceuticals feeding, the bacterial community did not return to its initial composition. Nevertheless, most of the assigned OTUs were present throughout the different operational phases. This core microbiome, represented by over 72% of the total sequences in each phase, probably played an important role in biological removal processes, avoiding their failure during the disturbance period. Quantitative-PCR revealed that pharmaceuticals load led to gradual changes on the abundance of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and polyphosphate-accumulating organisms (PAO) but their persistence during that phase demonstrated the resilience of such bacterial groups. AGS microbiome changed over time but a core community was maintained, probably ensuring the accomplishment of the main biological removal processes.
药品是经常存在于废水处理系统中的微量污染物。在这项研究中，研究了这些微量污染物对好氧颗粒污泥（AGS）生物反应器中细菌种群的潜在影响。通过 DGGE 指纹图谱和 barcoded pyrosequencing 分析，研究了 AGS 细菌群落结构和组成。这两种方法都揭示了一个动态的细菌群落的存在，而与药品的存在无关。AGS 微生物组在门和纲水平上随时间变化，停止给药剂后，细菌群落没有恢复到初始组成。然而，大多数分配的 OTUs 在不同的操作阶段都存在。这个核心微生物组，在每个阶段的总序列中占比超过 72%，可能在生物去除过程中发挥了重要作用，避免了在干扰期的失效。定量 PCR 显示，药剂负荷导致氨氧化菌（AOB）、亚硝酸盐氧化菌（NOB）和聚磷菌（PAO）的丰度逐渐变化，但它们在该阶段的持续存在证明了这些细菌群体的弹性。AGS 微生物组随时间变化，但维持一个核心群落，可能确保了主要生物去除过程的完成。
