钙黏蛋白-17和SATB同源盒蛋白2的联合检测可作为鉴别诊断肺肠型腺癌和转移性结直肠癌的潜在最佳标志物。
Combination of cadherin-17 and SATB homeobox 2 serves as potential optimal makers for the differential diagnosis of pulmonary enteric adenocarcinoma and metastatic colorectal adenocarcinoma.
作者信息
Bian Tingting, Zhao Jinli, Feng Jia, Zhang Qing, Qian Li, Liu Jian, Jiang Daishan, Liu Yifei, Zhang Jianguo
机构信息
Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China.
Department of Radiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, P.R. China.
出版信息
Oncotarget. 2017 Jun 28;8(38):63442-63452. doi: 10.18632/oncotarget.18828. eCollection 2017 Sep 8.
BACKGROUND
Pulmonary enteric adenocarcinoma (PEAC), a rare type of non-small cell lung cancer, has similar histological and immunohistochemical morphology to colorectal adenocarcinoma. Cadherin-17 (CDH17) and SATB homeobox 2 (SATB2) immunoexpression have recently been demonstrated in colorectal adenocarcinoma. In this study, we evaluated the value of CDH17 and SATB2 in the diagnosis of pulmonary enteric adenocarcinoma and metastatic colorectal adenocarcinoma.
METHODS
A total of 13 PEAC cases and 27 metastatic colorectal adenocarcinoma cases were enrolled in our cohort study. We analyzed the expressions of CK7, CK20, CDX-2, villin, cadherin-17 (CDH17), and SATB homeobox 2 (SATB2) using immunohistochemistry. Staining intensity and percentage of positive-staining cells were recorded. Sensitivity and specificity values for immunostains, individually and in combination, were computed and compared.
RESULTS
Combining CDH17 and SATB2 resulted in high sensitivity (76.92%) and specificity (100%). In our study, the use of CK7+, napsin A+, TTF-1+, napsin A+TTF-1+ in combination with CDH17-/SATB2- had a higher area under the curve compared to the combination CDH17-/SATB2-. However, no significant differences were observed between the combination CDH17-/SATB2- and other combinations (P>0.05).
CONCLUSIONS
In combination, CDH17 and SATB2 serve as potential optimal markers for the differential diagnosis of PEAC and metastatic colorectal adenocarcinoma.
背景
肺肠型腺癌（PEAC）是一种罕见的非小细胞肺癌，其组织学和免疫组化形态与结直肠腺癌相似。钙黏蛋白-17（CDH17）和SATB同源盒2（SATB2）免疫表达最近已在结直肠腺癌中得到证实。在本研究中，我们评估了CDH17和SATB2在肺肠型腺癌和转移性结直肠腺癌诊断中的价值。
方法
我们的队列研究共纳入了13例PEAC病例和27例转移性结直肠腺癌病例。我们使用免疫组化分析了细胞角蛋白7（CK7）、细胞角蛋白20（CK20）、尾型同源盒转录因子2（CDX-2）、绒毛蛋白、钙黏蛋白-17（CDH17）和SATB同源盒2（SATB2）的表达。记录染色强度和阳性染色细胞百分比。计算并比较免疫染色单独及联合使用时的敏感性和特异性值。
结果
联合使用CDH17和SATB2具有较高的敏感性（76.92%）和特异性（100%）。在我们的研究中，与CDH17-/SATB2-联合相比�，CK7+、天冬氨酸蛋白酶A+、甲状腺转录因子-1+、天冬氨酸蛋白酶A+甲状腺转录因子-1+联合CDH17-/SATB2-的曲线下面积更高。然而，CDH17-/SATB2-联合与其他联合之间未观察到显著差异（P>0.05）。
结论
联合使用时，CDH17和SATB2可作为肺肠型腺癌和转移性结直肠腺癌鉴别诊断的潜在最佳标志物。
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