Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
BMC Complement Altern Med. 2017 Oct 2;17(1):476. doi: 10.1186/s12906-017-1983-x.
Oxidative stress has a pivotal role in the pathogenesis and development of diabetic peripheral neuropathy (DPN), the most common and debilitating complications of diabetes mellitus. There is accumulating evidence that Juglans regia L. (GRL) leaf extract, a rich source of phenolic components, has hypoglycemic and antioxidative properties. This study aimed to determine the protective effects of Juglans regia L. leaf extract against streptozotocin-induced diabetic neuropathy in rat.
The DPN rat model was generated by intraperitoneal injection of a single 55 mg/kg dose of streptozotocin (STZ). A subset of the STZ-induced diabetic rats intragastically administered with GRL leaf extract (200 mg/kg/day) before or after the onset of neuropathy, whereas other diabetic rats received only isotonic saline as the same volume of GRL leaf extract. To evaluate the effects of GRL leaf extract on the diabetic neuropathy various parameters, including histopathology and immunohistochemistry of apoptotic and inflammatory factors were assessed along with nociceptive and biochemical assessments.
Degeneration of the sciatic nerves which was detected in the STZ-diabetic rats attenuated after GRL leaf extract administration. Greater caspase-3, COX-2, and iNOS expression could be detected in the STZ-diabetic rats, which were significantly attenuated after GRL leaf extract administration. Also, attenuation of lipid peroxidation and nociceptive response along with improved antioxidant status in the sciatic nerve of diabetic rats were detected after GRL leaf extract administration. In other word, GRL leaf extract ameliorated the behavioral and structural indices of diabetic neuropathy even after the onset of neuropathy, in addition to blood sugar reduction.
Our results suggest that GRL leaf extract exert preventive and curative effects against STZ-induced diabetic neuropathy in rats which might be due to its antioxidant, anti-inflammatory, and antiapoptotic properties. Protection against neuropathy.
氧化应激在糖尿病周围神经病变（DPN）的发病机制和发展中起着关键作用，DPN 是糖尿病最常见和最具致残性的并发症。有越来越多的证据表明，富含酚类成分的胡桃叶提取物具有降血糖和抗氧化作用。本研究旨在确定胡桃叶提取物对链脲佐菌素诱导的大鼠糖尿病神经病变的保护作用。
通过腹腔注射单次 55mg/kg 剂量的链脲佐菌素（STZ）生成 DPN 大鼠模型。一部分 STZ 诱导的糖尿病大鼠在神经病变发生前或发生后给予胡桃叶提取物（200mg/kg/天）灌胃，而其他糖尿病大鼠仅给予等渗盐水作为相同体积的胡桃叶提取物。为了评估胡桃叶提取物对糖尿病神经病变的影响，评估了各种参数，包括组织病理学和凋亡和炎症因子的免疫组织化学，以及痛觉和生化评估。
在 STZ 糖尿病大鼠中检测到的坐骨神经变性在给予胡桃叶提取物后减轻。在 STZ 糖尿病大鼠中可以检测到更高的 caspase-3、COX-2 和 iNOS 表达，给予胡桃叶提取物后这些表达明显减弱。此外，在给予胡桃叶提取物后，糖尿病大鼠坐骨神经中的脂质过氧化和痛觉反应减弱，抗氧化状态得到改善。换句话说，胡桃叶提取物改善了糖尿病神经病变的行为和结构指标，即使在神经病变发生后，血糖降低。
我们的结果表明，胡桃叶提取物对大鼠 STZ 诱导的糖尿病神经病变具有预防和治疗作用，这可能与其抗氧化、抗炎和抗凋亡特性有关。对神经病变的保护。
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