L. 叶提取物对大鼠糖尿病视网膜病变的治疗潜力
Therapeutic potential of L. leaf extract against diabetic retinopathy in rat.
作者信息
Nasiry Davood, Khalatbary Ali Reza, Ahmadvand Hassan
机构信息
Anatomy Department, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Iran J Basic Med Sci. 2017 Nov;20(11):1275-1281. doi: 10.22038/IJBMS.2017.9465.
OBJECTIVES
Oxidative stress has a pivotal role in the pathogenesis of diabetic retinopathy (DR). . (JRL) leaf extract has hypoglycemic and antioxidative properties. This study aimed to determine the ameliorative effects of JRL against diabetic retinopathy.
MATERIALS AND METHODS
The DR rat model was generated by injection of streptozotocin (STZ). A subset of the diabetic rats received JRL or metformin after the onset of hyperglycemia. Histopathology and immunohistochemistry of apoptotic and inflammatory factors were assessed along with biochemical assessments of lipid peroxidation and antioxidant status.
RESULTS
Lipid peroxidation level and catalase activity significantly improved after JRL consumption (<0. 001). Degeneration of the retina attenuated after JRL consumption. Attenuation of the caspase-3, COX-2, PARP, and S100B expression could be detected significantly (<0. 001) in the JRL-treated rats. While, blood glucose level decreased after JRL consumption (<0. 001).
CONCLUSION
JRL leaf extract exert protective effects against diabetic retinopathy.
目的
氧化应激在糖尿病视网膜病变（DR）的发病机制中起关键作用。（JRL）叶提取物具有降血糖和抗氧化特性。本研究旨在确定JRL对糖尿病视网膜病变的改善作用。
材料与方法
通过注射链脲佐菌素（STZ）建立DR大鼠模型。一部分糖尿病大鼠在高血糖发作后接受JRL或二甲双胍治疗。评估凋亡和炎症因子的组织病理学和免疫组织化学，以及脂质过氧化和抗氧化状态的生化评估。
结果
摄入JRL后脂质过氧化水平和过氧化氢酶活性显著改善（<0.001）。摄入JRL后视网膜变性减轻。在JRL治疗的大鼠中可显著检测到caspase-3、COX-2、PARP和S100B表达的减弱（<0.001）。同时，摄入JRL后血糖水平降低（<0.001）。
结论
JRL叶提取物对糖尿病视网膜病变具有保护作用。
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