Zeng Lanting, Liao Yinyin, Li Jianlong, Zhou Ying, Tang Jinchi, Dong Fang, Yang Ziyin
Guangdong Provincial Key Laboratory of Applied Botany & Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China; University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
Tea Research Institute, Guangdong Academy of Agricultural Sciences & Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Dafeng Road 6, Tianhe District, Guangzhou 510640, China.
Plant Sci. 2017 Nov;264:29-36. doi: 10.1016/j.plantsci.2017.08.005. Epub 2017 Aug 18.
Herbivore-induced plant volatiles (HIPVs) act as direct defenses against herbivores and as indirect defenses by attracting herbivore enemies. However, the involvement of HIPVs in within-plant or plant-to-plant signaling is not fully clarified. Furthermore, in contrast to model plants, HIPV signaling roles in crops have hardly been reported. Here, we investigated HIPVs emitted from tea (Camellia sinensis) plants, an important crop used for beverages, and their involvement in tea plant-to-plant signaling. To ensure uniform and sufficient exposure to HIPVs, jasmonic acid combined with mechanical damage (JAMD) was used to simulate herbivore attacks. Metabonomics techniques based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry and gas chromatography-mass spectrometry were employed to determine metabolite changes in undamaged tea plants exposed to JAMD-stimulated volatiles. JAMD-stimulated volatiles mainly enhanced the amounts of 1-O-galloyl-6-O-luteoyl-α-d-glucose, assamicain C, 2,3,4,5-tetrahydroxy-6-oxohexyl gallate, quercetagitrin, 2-(2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-8-yl)-4,5-dihydroxy-6-(hydroxymethyl)-tetrahydro-2H-pyran-3-yl, 3,4-dimethoxybenzoate, 1,3,4,5,6,7-hexahydroxyheptan-2-one, and methyl gallate in neighboring undamaged tea leaves. Furthermore, α-farnesene and β-ocimene, which were produced after JAMD treatments, were identified as two main JAMD-stimulated volatiles altering metabolite profiles of the neighboring undamaged tea leaves. This research advances our understanding of the ecological functions of HIPVs and can be used to develop crop biological control agents against pest insects in the future.
植食性昆虫诱导植物产生的挥发物（HIPVs）可作为对植食性昆虫的直接防御，并通过吸引植食性昆虫的天敌起到间接防御作用。然而，HIPVs在植物内部或植物间信号传导中的作用尚未完全阐明。此外，与模式植物相比，HIPVs在作物中的信号传导作用鲜有报道。在此，我们研究了作为重要饮料作物的茶树（Camellia sinensis）释放的HIPVs及其在茶树间信号传导中的作用。为确保对HIPVs有均匀且充足的暴露，采用茉莉酸联合机械损伤（JAMD）来模拟植食性昆虫的攻击。运用基于超高效液相色谱/四极杆飞行时间质谱和气相色谱 - 质谱的代谢组学技术，来测定未受损茶树在暴露于JAMD刺激挥发物后的代谢物变化。JAMD刺激挥发物主要增加了相邻未受损茶叶中1 - O - 没食子酰基 - 6 - O - 木犀草酰基 - α - D - 葡萄糖、阿萨姆卡因C、2,3,4,5 - 四羟基 - 6 - 氧代己基没食子酸酯、槲皮苷、2 - (2 - (3,4 - 二羟基苯基) - 5,7 - 二羟基 - 4 - 氧代 - 4H - 色烯 - 8 - 基) - 4,5 - 二羟基 - 6 - (羟甲基) - 四氢 - 2H - 吡喃 - 3 - 基、3,4 - 二甲氧基苯甲酸酯、1,3,4,5,6,7 - 六羟基庚烷 - 2 - 酮和没食子酸甲酯的含量。此外，JAMD处理后产生的α - 法尼烯和β - 罗勒烯被鉴定为改变相邻未受损茶叶代谢物谱的两种主要JAMD刺激挥发物。这项研究推进了我们对HIPVs生态功能的理解，并可用于未来开发针对害虫的作物生物防治剂。
