Stojsin-Carter Anja, Costa Nathalia N, De Morais Rodrigo, De Bem Tiago H, Costa Mayra P, Carter Timothy F, Gillis Daniel J, Neal Michael S, Ohashi Otavio M, Miranda Moyses S, Meirelles Flavio V, Favetta Laura A, King W Allan
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, 50 Stone Road, Guelph, ON, N1G 2W1, Canada.
Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa 1, Belém, PA, 66075-110, Brazil.
Anim Reprod Sci. 2017 Nov;186:85-92. doi: 10.1016/j.anireprosci.2017.09.010. Epub 2017 Sep 22.
Anti-Mullerian hormone (AMH) is expressed by both male and female fetuses during mammalian development, with males expressing AMH earlier and at significantly higher concentration. The aim of the current study was to explore the potential impact of pregnancy and fetal sex on maternal AMH and to determine if plasma (Pl) AMH or placenta intercotyledonary membrane and cotyledonary AMH receptor 2 (AMHR2) mRNA expression differ in pregnant cows carrying male vs. female fetuses. AMH levels in blood were measured using a bovine optimized ELISA kit. Cows pregnant with a male fetus were observed to have a significantly greater difference in Pl AMH between day 35 and 135 of gestation. Average fetal AMH level between 54 and 220days of gestation was also observed to be significantly higher in male vs. female fetuses. Intercotyledonary membranes and cotyledons were found to express AMHR2 between days 38 and 80 of gestation at similar levels in both fetal sexes. These findings support the hypothesis that fetal sex alters maternal Pl AMH during pregnancy in cattle.
抗苗勒管激素（AMH）在哺乳动物发育过程中由雄性和雌性胎儿表达，雄性表达AMH的时间更早且浓度显著更高。本研究的目的是探讨妊娠和胎儿性别对母体AMH的潜在影响，并确定携带雄性与雌性胎儿的怀孕母牛的血浆（Pl）AMH或胎盘叶间膜和绒毛叶AMH受体2（AMHR2）mRNA表达是否存在差异。使用牛优化ELISA试剂盒测量血液中的AMH水平。观察到怀有雄性胎儿的母牛在妊娠第35天至135天之间的Pl AMH差异显著更大。在妊娠54至220天之间，雄性胎儿的平均胎儿AMH水平也显著高于雌性胎儿。在妊娠第38天至80天之间，发现叶间膜和绒毛叶以相似水平表达AMHR2，且两种胎儿性别之间无差异。这些发现支持了胎儿性别会改变牛妊娠期间母体Pl AMH的假说。
