Ding Zhaoyang, Tan Jinyun, Feng Gang, Yuan Zhen, Wu Changfeng, Zhang Xuanjun
Faculty of Health Sciences , University of Macau , Macau SAR , China . Email:
Department of Biomedical Engineering , Southern University of Science and Technology , Shenzhen , Guangdong 518055 , China.
Chem Sci. 2017 Jul 1;8(7):5101-5106. doi: 10.1039/c7sc01077j. Epub 2017 May 18.
This work describes a facile yet powerful approach to energy-transfer NMOF (nanoscale metal-organic framework) fabrication for ratiometric peroxynitrite (ONOO) sensing. Poly(vinyl alcohol) (PVA) is chosen to organize the energy donor (NMOF) and acceptor (molecular probes). PVA can conveniently graft onto the NMOF surface and bind to the molecular probes bearing the arylboronic acid group through multiple weak coordination interactions. Due to efficient Förster resonance energy transfer (FRET), the bright blue fluorescence of the NMOF is quenched while the green or red emission from the acceptor is enhanced. Upon reacting with ONOO, the ONOO sensors depart from the NMOF and the FRET is interrupted and the fluorescence of the NMOF recovered. Based on this strategy, we developed two ratiometric ONOO nanosensors for the detection of ONOO in solutions and living cells. This work is the first report of NMOF ONOO sensors through FRET and could inspire the design of other NMOF based chemical sensors and biosensors.
这项工作描述了一种简便而强大的方法，用于制备用于比例式检测过氧亚硝酸盐（ONOO）的能量转移纳米级金属有机框架（NMOF）。选择聚乙烯醇（PVA）来组织能量供体（NMOF）和受体（分子探针）。PVA可以方便地接枝到NMOF表面，并通过多个弱配位相互作用与带有芳基硼酸基团的分子探针结合。由于高效的Förster共振能量转移（FRET），NMOF的亮蓝色荧光被淬灭，而受体发出的绿色或红色发射增强。与ONOO反应时，ONOO传感器从NMOF上脱离，FRET中断，NMOF的荧光恢复。基于此策略，我们开发了两种比例式ONOO纳米传感器，用于检测溶液和活细胞中的ONOO。这项工作是通过FRET制备NMOF ONOO传感器的首次报道，可能会激发其他基于NMOF的化学传感器和生物传感器的设计。
