Xu Ruoyu, Wang Youfu, Duan Xiaopin, Lu Kuangda, Micheroni Daniel, Hu Aiguo, Lin Wenbin
Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology , Shanghai 200237, China.
J Am Chem Soc. 2016 Feb 24;138(7):2158-61. doi: 10.1021/jacs.5b13458. Epub 2016 Feb 16.
We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.
我们报道了一种磷光/荧光双发射纳米级金属有机框架（NMOF）——R-UiO的设计，它可作为细胞内氧（O₂）传感器。R-UiO包含一个作为O₂敏感探针的Pt（II）-卟啉配体和一个作为O₂不敏感参考探针的罗丹明-B异硫氰酸酯配体。它具有良好的结晶性、高稳定性以及对O₂分压出色的比率发光响应。体外实验证实了R-UiO作为细胞内O₂生物传感器的适用性。这项工作是基于NMOF的细胞内氧传感器的首次报道，应能激发用于生物系统中其他重要分析物的比率NMOF传感器的设计。
