College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5;284:121827. doi: 10.1016/j.saa.2022.121827. Epub 2022 Sep 5.
Saxitoxin (STX) and tetrodotoxin (TTX) are widely distributed and extremely harmful marine toxins, it is certainly worth to spend effort to develop facile methods to detect them in sea food for human safety. In this work, two nano-sensors were developed by combining with two zirconium fluorescence Nanoscale metal-organic frameworks (NMOFs) with two emissions and TAMRA-labelled aptamers for STX and TTX sensing, respectively. The recognition of STX and TTX by these nano-sensors could change the structure of aptamer, which caused the blue or green emissions from NMOFs (energy donor) decreased while red emission from TAMRA-labelled aptamers (energy acceptor) increased owing to fluorescence resonance energy transfer (FRET) effect. Based on this strategy, NMOFs-Aptasensor 1 and NMOFs-Aptasensor 2 were developed for the ratiometric detection, with detection limits of 1.17 nM and 3.07 nM for STX and TTX, respectively. Moreover, NMOFs-Aptasensors displayed significant stability, pH-independence, selectivity and NMOFs-Aptasensors were successfully applied in shellfish sample for toxin sensing.
石房蛤毒素（STX）和河豚毒素（TTX）广泛分布且具有极强的危害性，因此值得投入精力开发简便的方法来检测海鲜中的这些毒素，以保障人类安全。在这项工作中，我们分别结合了两种具有两种发射光谱的锆荧光纳米级金属有机骨架（NMOFs）和 TAMRA 标记的适体，开发了两种纳米传感器，用于 STX 和 TTX 的传感。这些纳米传感器对 STX 和 TTX 的识别会改变适体的结构，从而导致来自 NMOFs（能量供体）的蓝色或绿色发射减少，而来自 TAMRA 标记的适体（能量受体）的红色发射增加，这是由于荧光共振能量转移（FRET）效应。基于这一策略，开发了用于比率检测的 NMOFs-Aptasensor 1 和 NMOFs-Aptasensor 2，其对 STX 和 TTX 的检测限分别为 1.17 nM 和 3.07 nM。此外，NMOFs-Aptasensors 表现出显著的稳定性、pH 独立性、选择性，并且成功应用于贝类样品中的毒素检测。
