Zhou Xiang, Michal Jennifer J, Jiang Zhihua, Liu Bang
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Department of Animal Sciences, Washington State University, Pullman, WA, 99164-6351, USA.
J Appl Genet. 2017 Nov;58(4):539-544. doi: 10.1007/s13353-017-0410-9. Epub 2017 Oct 2.
Porcine respiratory and reproductive syndrome (PRRS), caused by PRRS virus (PRRSV), is one of the most serious infectious diseases in the swine industry worldwide. Indigenous Chinese Tongcheng (TC) pigs reportedly show strong resistance to PRRSV infection. The miRNA expression profiles of porcine alveolar macrophages (PAMs) of control TC pigs and those infected with PRRSV in vivo were analyzed by high-throughput sequencing to explore changes induced by infection. A total of 182 known miRNAs including 101 miRNA-5p and 81 miRNA-3p were identified with 23 up-regulated differentially expressed miRNAs (DEmiRNAs) and 25 down-regulated DEmiRNAs. Gene Ontology analysis showed that predicted target genes for the DEmiRNAs were enriched in immune response, transcription regulation, and cell death. The integrative analysis of mRNA and miRNA expression revealed that down-regulated methylation-related genes (DNMT1 and DNMT3b) were targeted by five up-regulated DEmiRNAs. Furthermore, 35 pairs of miRNAs (70 miRNAs) were co-expressed after PRRSV infection and six pairs were co-expressed differently. Our results describe miRNA expression profiles of TC pigs in response to PRRSV infection and lay a strong foundation for developing novel therapies to control PRRS in pigs.
猪繁殖与呼吸综合征（PRRS）由猪繁殖与呼吸综合征病毒（PRRSV）引起，是全球养猪业中最严重的传染病之一。据报道，中国本土的桐城（TC）猪对PRRSV感染表现出强大的抵抗力。通过高通量测序分析了对照TC猪和体内感染PRRSV的猪的肺泡巨噬细胞（PAM）的miRNA表达谱，以探索感染引起的变化。共鉴定出182种已知的miRNA，包括101种miRNA-5p和81种miRNA-3p，其中有23种上调的差异表达miRNA（DEmiRNA）和25种下调的DEmiRNA。基因本体分析表明，DEmiRNA的预测靶基因在免疫反应、转录调控和细胞死亡方面富集。mRNA和miRNA表达的综合分析显示，五个上调的DEmiRNA靶向下调的甲基化相关基因（DNMT1和DNMT3b）。此外，PRRSV感染后有35对miRNA（70个miRNA）共表达，其中6对共表达存在差异。我们的研究结果描述了TC猪对PRRSV感染的miRNA表达谱，为开发控制猪PRRS的新疗法奠定了坚实基础。
