State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, P.R. China.
University of Chinese Academy of Sciences , Beijing 100049, P.R. China.
ACS Sens. 2017 Oct 27;2(10):1452-1457. doi: 10.1021/acssensors.7b00385. Epub 2017 Oct 17.
Diameter is a major concern for nanopore based sensing. However, directly pulling glass capillary nanopore with diameter down to sub-10 nm is very difficult. So, post treatment is sometimes necessary. Herein, we demonstrate a facile and effective wet-chemical method to shrink the diameter of glass capillary nanopore from several tens of nanometers to sub-10 nm by disodium silicate hydrolysis. Its benefits for DNA translocation are investigated. The shrinking of glass capillary nanopore not only slows down DNA translocation, but also enhances DNA translocation signal and signal-to-noise ratio significantly (102.9 for 6.4 nm glass nanopore, superior than 15 for a 3 nm silicon nitride nanopore). It also affects DNA translocation behaviors, making the approach and glass capillary nanopore platform promising for DNA translocation studies.
直径是基于纳米孔的传感的主要关注点。然而，直接将直径降至亚 10nm 的玻璃毛细管纳米孔拉伸非常困难。因此，有时需要进行后处理。在此，我们展示了一种简便有效的湿法化学方法，通过硅酸钠水解将玻璃毛细管纳米孔的直径从几十纳米缩小到亚 10nm。研究了其对 DNA 易位的益处。玻璃毛细管纳米孔的收缩不仅会减缓 DNA 易位，还会显著增强 DNA 易位信号和信噪比（6.4nm 玻璃纳米孔为 102.9，优于 3nm 氮化硅纳米孔的 15）。它还会影响 DNA 易位行为，使该方法和玻璃毛细管纳米孔平台成为 DNA 易位研究的有前途的方法。
