Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, and ‡Department of Chemistry, University of Helsinki , Helsinki, 00014 Finland.
Anal Chem. 2017 Nov 7;89(21):11214-11218. doi: 10.1021/acs.analchem.7b01638. Epub 2017 Oct 13.
We present a new high-throughput platform for studying titanium dioxide (TiO) photocatalytic oxidation reactions by performing reactions on a TiO-coated surface, followed by direct analysis of oxidation products from the surface by desorption electrospray ionization mass spectrometry (DESI-MS). For this purpose, we coated a round glass wafer with photocatalytically active anatase-phase TiO using atomic layer deposition. Approximately 70 aqueous 1 μL samples can be injected onto the rim of the TiO-coated glass wafer, before the entire wafer is exposed to UV irradiation. After evaporation of water, the oxidation products can be directly analyzed from the sample spots by DESI-MS, using a commercial rotating sample platform. The method was shown to provide fast photocatalytic oxidation reactions and analysis with throughput of about four samples per minute. The feasibility of the method was examined for mimicking phase I metabolism reactions of amodiaquine, buspirone and verapamil. Their main photocatalytic reaction products were mostly similar to the products observed earlier in TiO photocatalysis and in in vitro phase I metabolism assays performed using human liver microsomes.
我们提出了一种新的高通量平台，用于通过在 TiO 涂层表面进行反应，然后通过解吸电喷雾电离质谱（DESI-MS）直接分析表面的氧化产物来研究二氧化钛（TiO）光催化氧化反应。为此，我们使用原子层沉积在圆形玻璃晶片上涂覆了具有光催化活性的锐钛矿相 TiO。可以将大约 70 种水性 1 μL 样品注入到 TiO 涂层玻璃晶片的边缘，然后再将整个晶片暴露于 UV 照射下。水蒸发后，通过 DESI-MS 可以直接从样品点分析氧化产物，使用商业旋转样品平台。该方法被证明能够提供快速的光催化氧化反应和分析，每分钟约有四个样品的通量。该方法的可行性通过模拟阿莫地喹、丁螺环酮和维拉帕米的 I 相代谢反应进行了检验。它们的主要光催化反应产物与 TiO 光催化和使用人肝微粒体进行的体外 I 相代谢测定中观察到的产物相似。
