Urinary alanine aminopeptidase assay improved as result of multivariate response-surface analysis.
作者信息
Flandrois C, Lahet C, Feldmann D, Gabastou J M, Gonnon A, Maire I
机构信息
Laboratoire d'Enzymologie (Monique Mathieu), Hôpital Debrousse, Lyon, France.
出版信息
Clin Chem. 1988 May;34(5):954-7.
PMID:2897258
Abstract
Optimization of determination of alanine aminopeptidase in urine by univariate study led to a method involving pretreatment of urine with Sephadex G50. Re-examination of the optimization by multivariate study led us to recommend higher optimal concentrations: 5.8 mmol/L for the substrate and 300 mmol/L for the Tris buffer. Under these new conditions, pretreatment of urine was no longer necessary and the assay could be completely automated.
摘要
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