Wu Liancheng, Li Mingna, Tian Lei, Wang Shunxi, Wu Liuji, Ku Lixia, Zhang Jun, Song Xiaoheng, Liu Haiping, Chen Yanhui
College of Agronomy, Synergetic Innovation Centre of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, China.
3Cereal Institute, Henan Academy of Agricultural Sciences/Henan Provincial Key Laboratory of Maize Biology, Zhengzhou, China.
PLoS One. 2017 Oct 3;12(10):e0185838. doi: 10.1371/journal.pone.0185838. eCollection 2017.
In maize (Zea mays), leaf senescence acts as a nutrient recycling process involved in proteins, lipids, and nucleic acids degradation and transport to the developing sink. However, the molecular mechanisms of pre-maturation associated with pollination-prevention remain unclear in maize. To explore global gene expression changes during the onset and progression of senescence in maize, the inbred line 08LF, with severe early senescence caused by pollination prevention, was selected. Phenotypic observation showed that the onset of leaf senescence of 08LF plants occurred approximately 14 days after silking (DAS) by pollination prevention. Transcriptional profiling analysis of the leaf at six developmental stages during induced senescence revealed that a total of 5,432 differentially expressed genes (DEGs) were identified, including 2314 up-regulated genes and 1925 down-regulated genes. Functional annotation showed that the up-regulated genes were mainly enriched in multi-organism process and nitrogen compound transport, whereas down-regulated genes were involved in photosynthesis. Expression patterns and pathway enrichment analyses of early-senescence related genes indicated that these DEGs are involved in complex regulatory networks, especially in the jasmonic acid pathway. In addition, transcription factors from several families were detected, particularly the CO-like, NAC, ERF, GRAS, WRKY and ZF-HD families, suggesting that these transcription factors might play important roles in driving leaf senescence in maize as a result of pollination-prevention.
在玉米（Zea mays）中，叶片衰老作为一种营养物质循环过程，涉及蛋白质、脂质和核酸的降解以及向发育中的库的转运。然而，玉米中与授粉阻止相关的早熟分子机制仍不清楚。为了探究玉米衰老起始和进程中的全基因组表达变化，选择了因授粉阻止导致严重早期衰老的自交系08LF。表型观察表明，08LF植株的叶片衰老起始发生在授粉后大约14天的吐丝期（DAS）。对诱导衰老过程中六个发育阶段的叶片进行转录谱分析，结果显示共鉴定出5432个差异表达基因（DEG），其中包括2314个上调基因和1925个下调基因。功能注释表明，上调基因主要富集在多生物体过程和氮化合物转运中，而下调基因则参与光合作用。对早期衰老相关基因的表达模式和通路富集分析表明，这些差异表达基因参与复杂的调控网络，尤其是茉莉酸途径。此外，还检测到几个家族的转录因子，特别是CO-like、NAC、ERF、GRAS、WRKY和ZF-HD家族，这表明这些转录因子可能在授粉阻止导致的玉米叶片衰老中发挥重要作用。
