Yuhki N, O'Brien S J
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21701-1013.
Immunogenetics. 1988;27(6):414-25. doi: 10.1007/BF00364427.
The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from man and mouse. The cat possesses a minimum of 20 class I loci and 5 class II genes per haploid genome. Class I genes of the domestic cat expressed limited restriction fragment length polymorphism. The average percent difference of the size of DNA fragments between individual cats was 9.0%, a value five times lower than the value for mice, but comparable to the human DNA polymorphism level. Class I and class II genes were both genetically mapped to feline chromosome B2 using a panel of rodent x cat somatic cell hybrids. Since feline chromosome B2 is syntenically homologous to human chromosome 6 and mouse chromosome 17, these results affirm the linkage conservation of the MHC-containing linkage group in the three mammalian orders.
家猫的主要组织相容性复合体（MHC）至今尚未得到充分表征，主要原因是制备同种异型血清存在诸多困难。我们在此展示了利用人和小鼠的MHC分子探针，对家猫群体中的I类和II类基因进行的比较分析。家猫每个单倍体基因组至少拥有20个I类基因座和5个II类基因。家猫的I类基因表现出有限的限制性片段长度多态性。个体猫之间DNA片段大小的平均差异百分比为9.0%，该值比小鼠的值低五倍，但与人类DNA多态性水平相当。利用一组啮齿动物x猫体细胞杂种，将I类和II类基因都定位到了猫的B2染色体上。由于猫的B2染色体与人类6号染色体和小鼠17号染色体在同线性上是同源的，这些结果证实了三个哺乳动物目中含MHC的连锁群的连锁保守性。
