González-Robles Arturo, Omaña-Molina Maritza, Salazar-Villatoro Lizbeth, Flores-Maldonado Catalina, Lorenzo-Morales Jacob, Reyes-Batlle María, Arnalich-Montiel Francisco, Martínez-Palomo Adolfo
Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Mexico City, Mexico.
Faculty of Superior Studies, UNAM, Iztacala, Tlalnepantla, State of Mexico, Mexico.
Exp Parasitol. 2017 Dec;183:245-253. doi: 10.1016/j.exppara.2017.09.018. Epub 2017 Sep 30.
Acanthamoeba culbertsoni trophozoites, previously isolated from a human keratitis case with severe intraocular damage, were maintained in axenic culture. Co-incubation of amoebae with MDCK cell monolayers demonstrated an apparent preference of the amoebae to introduce themselves between the cells. The trophozoites appeared to cross the cell monolayer through the tight junctions, which resulted in decreased trans-epithelial resistance (TER) measurements. Unexpectedly, after co-incubation of amoebae with hamster corneas, we observed that the trophozoites were able to cross the different cell layers and reach the corneal stroma after only 12 h of interaction, in contrast to other Acanthamoeba species. These observations suggest that this A. culbertsoni isolate is particularly pathogenic. Further research with diverse methodologies needs to be performed to explain the unique behavior of this Acanthamoeba strain.
曾从一例伴有严重眼内损伤的人类角膜炎病例中分离出的库氏棘阿米巴滋养体，在无菌培养条件下进行培养。将阿米巴与MDCK细胞单层共同孵育后发现，阿米巴明显倾向于在细胞之间侵入。滋养体似乎通过紧密连接穿过细胞单层，这导致跨上皮电阻（TER）测量值降低。出乎意料的是，在将阿米巴与仓鼠角膜共同孵育后，我们观察到，与其他棘阿米巴物种不同，仅经过12小时的相互作用，滋养体就能穿过不同的细胞层并到达角膜基质。这些观察结果表明，这种库氏棘阿米巴分离株具有特别强的致病性。需要采用多种方法进行进一步研究，以解释这种棘阿米巴菌株的独特行为。
