Behringer Richard, Gertsenstein Marina, Nagy Kristina Vintersten, Nagy Andras
Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot092627. doi: 10.1101/pdb.prot092627.
In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracycline-inducible system to control the expression of the Yamanaka reprogramming factors.
2006年，山中伸弥及其学生高桥和利表明，仅四个特定基因的表达就足以将完全分化的体细胞重编程为多能干细胞。这些细胞被称为诱导多能干细胞（iPS细胞），它们与胚胎干细胞（ES细胞）具有许多共同特征。在本实验方案中，我们描述了从小鼠成纤维细胞生成iPS细胞的最简单方法之一。它结合了高效的转座子介导转染和四环素诱导系统来控制山中重编程因子的表达。
