Behringer Richard, Gertsenstein Marina, Nagy Kristina Vintersten, Nagy Andras
Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot092619. doi: 10.1101/pdb.prot092619.
Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors-OCT4, SOX2, KLF4, and c-MYC. The transposon system has proven effective as a vehicle for the delivery of transgenes into fibroblasts and for successful reprogramming to induced pluripotent stem (iPS) cells. We found that FuGENE HD transfection reagent can be effective for mouse embryonic fibroblasts (MEFs) to generate induced pluripotent stem cells (iPSCs) with the transposon transgenes. There are multitudes of cell transfection methods commercially available. Their efficiency, and thus their success, in inducing pluripotent cell types are cell-type-dependent.
通过强制表达四种转录因子——OCT4、SOX2、KLF4和c-MYC，小鼠胚胎成纤维细胞可被重编程为胚胎干细胞样多能干细胞。转座子系统已被证明是一种有效的载体，可用于将转基因导入成纤维细胞，并成功重编程为诱导多能干细胞（iPS细胞）。我们发现，FuGENE HD转染试剂对于小鼠胚胎成纤维细胞（MEF）利用转座子转基因生成诱导多能干细胞（iPSC）可能是有效的。市面上有大量的细胞转染方法。它们在诱导多能细胞类型方面的效率以及成功率取决于细胞类型。
