Green Michael R, Sambrook Joseph
Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093427. doi: 10.1101/pdb.prot093427.
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles is limited. Cells infected with bacteriophage M13 are not killed, but have a longer generation time than uninfected In consequence, plaques appear as areas of slower-growing cells on a faster-growing lawn of bacterial cells. This protocol describes plating of bacteriophage M13 stocks. Plaques are readily detectable on top agar after 4-8 h of incubation at 37°C.
M13噬菌体噬菌斑源自单个病毒粒子对单个细菌的感染。子代粒子感染邻近细菌，这些细菌继而释放出另一代子代病毒粒子。如果细菌在半固体培养基（如含有琼脂或琼脂糖的培养基）中生长，那么子代粒子的扩散就会受到限制。被M13噬菌体感染的细胞不会被杀死，但与未感染的细胞相比，其世代时间更长。因此，噬菌斑在生长较快的细菌细胞菌苔上表现为生长较慢的细胞区域。本实验方案描述了M13噬菌体原种的铺板操作。在37°C孵育4 - 8小时后，在顶层琼脂上很容易检测到噬菌斑。
