Tumor Hospital of Yunnan Province, Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(17):3959-3965.
The purpose of this project was to examine the effects of estradiol (E2) and progesterone (P) on the proliferation of HeLa cells. E2 promoted and P inhibited the proliferation of HeLa cells.
Then, we tested the consequence of combining the different activities of E2 + P.
We found that P inhibited the proliferative effect of E2 on HeLa cells. Analysis of fotocitometry (FCM) cell cycle and apoptosis rate showed that E2 decreased significantly G0/G1 phase, P and E2 + P significantly increased G0/G, and decreased significantly S phase. P inhibits tumor growth and induces apoptosis mainly by blocking the progression from G1 to S phase. Thus, the E2 + P combination can effectively inhibit the effect of E2 on HeLa cell proliferation. Meanwhile, the E2 + P combination can block the progression from G1 to S phase to induce apoptosis.
Overall, these results suggest that the combined application of E2 and P can be a new effective strategy for hormone replacement therapy after treatment of cervical adenocarcinoma.
本项目旨在研究雌二醇（E2）和孕酮（P）对 HeLa 细胞增殖的影响。E2 促进，P 抑制 HeLa 细胞增殖。
然后，我们测试了 E2+P 不同活性的联合作用的结果。
我们发现 P 抑制了 E2 对 HeLa 细胞的增殖作用。细胞周期和凋亡率的 fotocitometry（FCM）分析表明，E2 显著降低 G0/G1 期，P 和 E2+P 显著增加 G0/G1 期，S 期显著降低。P 主要通过阻止 G1 期向 S 期的进展来抑制肿瘤生长并诱导凋亡。因此，E2+P 联合可以有效抑制 E2 对 HeLa 细胞增殖的作用。同时，E2+P 联合可以阻断 G1 期向 S 期的进展，诱导细胞凋亡。
总的来说，这些结果表明，E2 和 P 的联合应用可能是宫颈癌治疗后激素替代治疗的一种新的有效策略。
