Department of Rheumatology, Yantaishan Hospital, Yantai, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(17):3879-3885.
This study sought to explore the correlation between IL-4-590C/T polymorphism as well as mRNA expression and the occurrence of rheumatoid arthritis (RA).
IL-4-590C/T polymorphisms, detected by a TaqMan probe from 150 RA patients who were treated in Yantaishan Hospital from June 2014 to June 2016, and from 150 healthy people, all from the Han population, were selected for the study. Interleukin-4 (IL-4) mRNA expression was detected by Real-time Polymerase Chain Reaction (PCR), and the differences in IL-4mRNA expressions of different genotypes in RA patients were compared.
The difference in CC, CT and TT genotype distributions between the RA group and the healthy group were statistically significant (p<0.05). The mutation frequency of the T allele in IL-4 of the RA group was significantly higher than that of the healthy group (p<0.01). The mRNA expression of IL-4 in the RA group was significantly lower than that in the healthy group (p<0.01). The mRNA expression of IL-4 in RA patients was gradually decreased in the following order: CC, CT and TT, and the differences among these genotypes were significant (p<0.01).
IL-4-590C/T polymorphism may be correlated with the incidence of RA in the Chinese Han population. Carrying the T allele can significantly increase the risk of RA and reduce the mRNA expression of IL-4.
本研究旨在探讨白细胞介素 4（IL-4）-590C/T 多态性及其 mRNA 表达与类风湿关节炎（RA）发生的相关性。
选择 2014 年 6 月至 2016 年 6 月在烟台市烟台山医院治疗的 150 例 RA 患者和 150 例健康人群（均为汉族），采用 TaqMan 探针检测 IL-4-590C/T 多态性。采用实时聚合酶链反应（PCR）检测白细胞介素 4（IL-4）mRNA 表达，比较不同基因型 RA 患者 IL-4mRNA 表达的差异。
RA 组与健康组 CC、CT 和 TT 基因型分布差异有统计学意义（p<0.05）。RA 组 IL-4 的 T 等位基因突变频率明显高于健康组（p<0.01）。RA 组 IL-4mRNA 表达明显低于健康组（p<0.01）。RA 组 IL-4mRNA 表达逐渐降低，CC、CT 和 TT 基因型依次降低，差异均有统计学意义（p<0.01）。
IL-4-590C/T 多态性可能与中国汉族人群 RA 的发病有关。携带 T 等位基因可显著增加 RA 的发病风险，降低 IL-4mRNA 表达。
