SIHMDS Haematology, Camelia Botnar Laboratories, Great Ormond Street Hospital for Children NHS Trust, London, UK.
Immunology, Camelia Botnar Laboratories, Great Ormond Street Hospital for Children NHS Trust, London, UK.
Clin Exp Immunol. 2018 Feb;191(2):198-202. doi: 10.1111/cei.13062. Epub 2017 Oct 27.
Assessment of thymic output by measurement of naive T cells is carried out routinely in clinical diagnostic laboratories, predominantly using flow cytometry with a suitable panel of antibodies. Naive T cell measurements can also be made using molecular analyses to quantify T cell receptor excision circle (TRECs) levels in sorted cells from peripheral blood. In this study we have compared TRECs levels retrospectively with CD45RA CD27 T cells and also with CD45RA CD31 T cells in 134 patient samples at diagnosis or during follow-up. Both panels provide naive T cell measurements that have a strongly positive correlation with TRECs numbers and are suitable for use with enumerating naive T cell levels in a clinical laboratory.
通过测量幼稚 T 细胞来评估胸腺输出在临床诊断实验室中是常规进行的，主要使用流式细胞术和适当的抗体组合。幼稚 T 细胞的测量也可以使用分子分析来定量外周血分选细胞中的 T 细胞受体切除环 (TRECs) 水平。在这项研究中，我们回顾性地比较了 134 个患者样本在诊断或随访时的 TRECs 水平与 CD45RA CD27 T 细胞和 CD45RA CD31 T 细胞。这两个面板都提供了与 TRECs 数量具有强烈正相关的幼稚 T 细胞测量值，适用于在临床实验室中计数幼稚 T 细胞水平。
