生物活性苏糖-D-异柠檬酸对尾草履虫氧化应激影响的研究。
Investigation of the effect of biologically active threo-Ds-isocitric acid on oxidative stress in Paramecium caudatum.
作者信息
Morgunov Igor G, Karpukhina Olga V, Kamzolova Svetlana V, Samoilenko Vladimir A, Inozemtsev Anatoly N
机构信息
a G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms , Russian Academy of Sciences , Pushchino , Russia.
b Department of Higher Nervous Activity, Faculty of Biology , Lomonosov Moscow State University , Moscow , Russia.
出版信息
Prep Biochem Biotechnol. 2018 Jan 2;48(1):1-5. doi: 10.1080/10826068.2017.1381622. Epub 2018 Jan 3.
The effect of biologically active form (threo-Ds-) of isocitric acid (ICA) on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). ICA at concentrations between 0.5 and 10 mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of ICA was observed at its concentration 10 mM irrespective of the toxicant used (either HO or heavy metal ions). ICA was found to be a more active antioxidant than ascorbic acid. Biologically active pharmaceutically pure threo-Ds-ICA was produced through cultivation of the yeast Yarrowia lipolytica and isolated from the culture liquid in the form of crystalline monopotassium salt with a purity of 99.9%.
利用过氧化氢和某些重金属（铜、铅、锌和镉）盐胁迫下的尾草履虫，研究了生物活性形式（苏阿糖-Ds-）异柠檬酸（ICA）对氧化应激的影响。浓度在0.5至10 mM之间的ICA对所研究的毒物诱导的氧化应激的尾草履虫细胞有有利影响。无论使用何种毒物（过氧化氢或重金属离子），在10 mM浓度下观察到ICA的最大抗氧化作用。发现ICA是比抗坏血酸更具活性的抗氧化剂。通过解脂耶氏酵母培养生产生物活性药用纯苏阿糖-Ds-ICA，并以纯度为99.9%的结晶单钾盐形式从培养液中分离出来。
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