Kamzolova Svetlana V, Samoilenko Vladimir A, Lunina Julia N, Morgunov Igor G
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, Prospect Nauki 5, Pushchino 142290, Moscow Region, Russia.
BioTech (Basel). 2023 Mar 13;12(1):22. doi: 10.3390/biotech12010022.
Isocitric acid (ICA) refers to a group of promising regulators of energy metabolism which has antistress, antihypoxic, and antioxidant activities. In this paper, we reported a process of ICA production from rapeseed oil using yeast VKM Y-2373 in a 500-L fermentor. The producer synthesized 64.1 g/L ICA with a product yield of 0.72 g/g and a productivity 0.54 g/L·h. We also developed an effective purification method, including a cell separation, clarification, concentration, acidification, and crystallization process, which resulted in the formation of the crystals of monopotassium salt of ICA with a purity of 99.0-99.9%. To the best of our knowledge, this is the first report on an ICA production process at an upscaled bioreactor level.
异柠檬酸（ICA）是一类具有抗应激、抗缺氧和抗氧化活性的、很有前景的能量代谢调节剂。在本文中，我们报道了在500升发酵罐中使用酵母VKM Y-2373从菜籽油生产ICA的过程。该生产菌株合成了64.1克/升的ICA，产物得率为0.72克/克，生产效率为0.54克/升·小时。我们还开发了一种有效的纯化方法，包括细胞分离、澄清、浓缩、酸化和结晶过程，最终得到了纯度为99.0 - 99.9%的ICA单钾盐晶体。据我们所知，这是关于在放大的生物反应器水平上生产ICA过程的首次报道。
