Center for Optical Metrology, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
J Biophotonics. 2018 Feb;11(2). doi: 10.1002/jbio.201700174. Epub 2017 Oct 29.
Two-photon nonlinear microscopy with the aid of plasmonic contrast agents is an attractive bioimaging technique capable of generating high-resolution images in 3 dimensions and facilitating targeted imaging with deep tissue penetration. In this work, physically synthesized gold nanoparticles containing multiple nanopores are used as 2-photon contrast agents and are reported to emit a 20-fold brighter 2-photon luminescence as compared to typical contrast agents, that is, gold nanorods. A successful application of our porous gold nanoparticles is experimentally demonstrated by in vitro nonlinear optical imaging of adipocytes at subcellular level.
基于等离子体对比剂的双光子非线性显微镜是一种很有吸引力的生物成像技术，它能够在 3 维空间中生成高分辨率的图像，并实现具有深层组织穿透能力的靶向成像。在这项工作中，我们使用物理合成的含有多个纳米孔的金纳米粒子作为双光子对比剂，据报道，与典型的对比剂（即金纳米棒）相比，其双光子荧光强度增强了 20 倍。我们的多孔金纳米粒子的成功应用通过亚细胞水平的脂肪细胞的体外非线性光学成像实验得到了验证。
