Rogler J C, de Moraes G H, Elkin R G
Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907.
Proc Soc Exp Biol Med. 1988 Jun;188(2):169-72. doi: 10.3181/00379727-188-42723.
The incorporation of [14C]glutamic acid into EDTA-soluble and -insoluble calvaria protein in vitro and [14C]proline into EDTA-insoluble femur protein in vivo was determined in chicks fed inadequate and adequate levels of nonspecific nitrogen (glutamic acid). In each instance, the amount of amino acid incorporated into bone protein was reduced by the low level of nonspecific nitrogen. It was concluded that the high incidence of leg abnormalities observed in chicks fed purified diets containing adequate levels of indispensable amino acids but lacking in total nitrogen might be associated with an inability to form bone matrix protein.
在体外测定了喂食非特异性氮（谷氨酸）水平不足和充足的雏鸡中，[14C]谷氨酸掺入颅骨EDTA可溶性和不溶性蛋白质的情况，以及在体内测定了[14C]脯氨酸掺入股骨EDTA不溶性蛋白质的情况。在每种情况下，非特异性氮水平低都会降低掺入骨蛋白的氨基酸量。得出的结论是，在喂食含有充足必需氨基酸但总氮缺乏的纯化日粮的雏鸡中观察到的腿部异常高发生率，可能与无法形成骨基质蛋白有关。
