Jankásková B, Lisý V, Stastný F
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Physiol Bohemoslov. 1988;37(1):19-25.
The development of gamma-glutamyl transpeptidase (GGT) activity in neurones and glial cells was studied in primary cell cultures derived from the cerebral hemispheres of chick embryos. GGT activity was found in both basic types of nervous tissue cells. It was always higher in glial cell cultures, where it was up to 2.3-fold the values in neurone-enriched cultures. If the culture medium contained foetal calf serum, the GGT activity of both types of nerve cells was higher than in the presence of inactivated calf serum. Comparison with the in vivo situation showed that the level of GGT activity in nerve cell cultures was significantly lower. Between the seventh day of embryogenesis and the third day of postnatal development of the nerve cells, there were marked differences between the GGT activity of cells maintained under in vitro conditions and cells of the same age in brain tissue homogenate. GGT activity in cerebral hemisphere homogenates from a 17-day-old embryos amounted to 4-fold the activity in a primary glial cell culture and to 16-fold the value in a neurone-enriched culture from hemispheres at the same stage of development.
在源自鸡胚大脑半球的原代细胞培养物中，研究了神经元和神经胶质细胞中γ-谷氨酰转肽酶（GGT）活性的发育情况。在两种基本类型的神经组织细胞中均发现了GGT活性。在神经胶质细胞培养物中，其活性总是更高，最高可达富含神经元的培养物中活性值的2.3倍。如果培养基中含有胎牛血清，两种类型神经细胞的GGT活性均高于含有热灭活小牛血清的情况。与体内情况相比，神经细胞培养物中GGT活性水平显著更低。在胚胎发育的第7天至神经细胞出生后发育的第3天之间，体外培养条件下的细胞与相同年龄脑组织匀浆中的细胞在GGT活性方面存在显著差异。17日龄胚胎大脑半球匀浆中的GGT活性是原代神经胶质细胞培养物中活性的4倍，是同一发育阶段半球富含神经元培养物中活性值的16倍。
