Vernadakis A, Estin C, Gibson D A, Amott S
J Neurosci Res. 1982;7(2):111-7. doi: 10.1002/jnr.490070203.
Mixed neuronal and nonneuronal cell cultures were obtained from 8-day-old chick embryos cerebral hemispheres and glial-enriched cultures were obtained from fifteen-day-old chick embryo cerebral hemispheres. Cultures were exposed to methadone, a narcotic drug, from days four to six. The activity of ornithine decarboxylase (ODC) was determined at day eight and the activity of cyclic nucleotide phosphohydrolase (CNP) was determined at day fifteen. Both ODC and CNP activity were higher in mixed neuronal-nonneuronal cell cultures treated with methadone as compared to control. No effect was observed in the neuronal-enriched or glial-enriched cultures. These findings are interpreted to reflect that neuronal-glial interaction is important in the response of primary neural cells to methadone.
混合神经元和非神经元细胞培养物取自8日龄鸡胚的大脑半球，富含神经胶质细胞的培养物取自15日龄鸡胚的大脑半球。培养物在第4天至第6天暴露于麻醉药物美沙酮。在第8天测定鸟氨酸脱羧酶（ODC）的活性，在第15天测定环核苷酸磷酸水解酶（CNP）的活性。与对照组相比，用美沙酮处理的混合神经元-非神经元细胞培养物中ODC和CNP的活性均较高。在富含神经元或富含神经胶质细胞的培养物中未观察到影响。这些发现被解释为反映了神经元-神经胶质细胞相互作用在原代神经细胞对美沙酮的反应中很重要。
