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[醋酸纤维素电泳后γ-谷氨酰转移酶多种分子形式测定的新方法。初步数据]

[A new method for the determination of the multiple molecular forms of gamma-glutamyltransferases after electrophoresis on cellulose acetate. Preliminary data].

作者信息

Rasini G, Bertolini G, Caprio G

机构信息

Laboratorio di analisi chimico-cliniche e microbiologiche, Ospedale Fatebenefratelli ed Oftalmico, Milano.

出版信息

Quad Sclavo Diagn. 1987 Jun;23(2):123-31.

PMID:2897702
Abstract

What is described is a new, sensitive, simple and precise method for the visualization of the multiple molecular forms of the gamma-glutamyltransferase by means of electrophoresis on cellulose acetate in parallel with the serum protein pattern. The isoenzymatic pattern in 100 cases with a normal catalytic activity was mainly represented by two bands, one in the alpha 1 region and one in the alpha 2. Also examined was the serum of 103 cases having various hepatic biliary pathologies and a catalytic activity out of the reference interval, in an attempt to evaluate the diagnostic usefulness of the various isoenzyme patterns.

摘要

本文描述了一种新的、灵敏、简单且精确的方法,用于通过在醋酸纤维素上进行电泳并与血清蛋白图谱平行,来可视化γ-谷氨酰转移酶的多种分子形式。100例催化活性正常的病例中,同工酶图谱主要由两条带代表,一条在α1区域,一条在α2区域。还检测了103例患有各种肝胆疾病且催化活性超出参考区间的病例的血清,以评估各种同工酶图谱的诊断效用。

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