Sacchetti L, Castaldo G, Fortunato G, Salvatore F
Istituto di Scienze Biochimiche, IIa Facoltà di Medicina, Università di Napoli, Italy.
Clin Chem. 1988 Feb;34(2):419-22.
In this electrophoretic procedure for measuring isoenzymes of gamma-glutamyltransferase, patterns obtained are highly reproducible and the analytical imprecision (CV) ranges from 1.10% to 6.17%. A cellulose acetate support is used, at 220 V for 40 min. Sharply resolved isoenzyme bands were made visible by fluorescence scattered light, formed by use of a coumarin derivative as donor substrate. Two bands were observed for sera from normal subjects; four bands were variably present in sera from patients with different hepatobiliary diseases. Detection of the latter was satisfactory, down to a total activity in serum of 8-10 U/L. Three of the pathological bands were associated with low- and (or) very-low-density lipoproteins, whereas a major fraction of one of the normal bands in cirrhotic sera precipitated with high-density lipoprotein. The bands in normal sera, and one of the abnormal bands, did not.
在这种用于测量γ-谷氨酰转移酶同工酶的电泳程序中，所获得的图谱具有高度可重复性，分析不精密度（CV）范围为1.10%至6.17%。使用醋酸纤维素支持物，在220V下电泳40分钟。通过使用香豆素衍生物作为供体底物形成的荧光散射光使清晰分辨的同工酶带可见。正常受试者血清观察到两条带；不同肝胆疾病患者的血清中可变地出现四条带。对后者的检测效果良好，血清总活性低至8 - 10U/L。三条病理带与低密度和（或）极低密度脂蛋白相关，而肝硬化血清中一条正常带的主要部分与高密度脂蛋白沉淀。正常血清中的带以及一条异常带则不会。
