Tempesta Camille, Hijazi Assia, Moussian Bernard, Roch Fernando
Centre de Biologie du Développement (CBD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, France.
Lebanese University, Faculty of Sciences I and V-Doctorate School of Science and Technology-PRASE, Campus Rafic Hariri, Hadath-Beirut, Lebanon.
PLoS One. 2017 Oct 4;12(10):e0185897. doi: 10.1371/journal.pone.0185897. eCollection 2017.
The maintenance of paracellular barriers in invertebrate epithelia depends on the integrity of specific cell adhesion structures known as septate junctions (SJ). Multiple studies in Drosophila have revealed that these junctions have a stereotyped architecture resulting from the association in the lateral membrane of a large number of components. However, little is known about the dynamic organisation adopted by these multi-protein complexes in living tissues. We have used live imaging techniques to show that the Ly6 protein Boudin is a component of these adhesion junctions and can diffuse systemically to associate with the SJ of distant cells. We also observe that this protein and the claudin Kune-kune are endocytosed in epidermal cells during embryogenesis. Our data reveal that the SJ contain a set of components exhibiting a high membrane turnover, a feature that could contribute in a tissue-specific manner to the morphogenetic plasticity of these adhesion structures.
无脊椎动物上皮细胞旁细胞屏障的维持取决于特定细胞黏附结构（即分隔连接，SJ）的完整性。果蝇的多项研究表明，这些连接具有由大量成分在侧膜中缔合形成的固定结构。然而，对于这些多蛋白复合物在活组织中采用的动态组织方式知之甚少。我们利用实时成像技术表明，Ly6蛋白Boudin是这些黏附连接的一个成分，并且可以全身扩散以与远处细胞的SJ缔合。我们还观察到，在胚胎发育过程中，这种蛋白质和claudin Kune-kune在表皮细胞中被内吞。我们的数据表明，SJ包含一组具有高膜更新率的成分，这一特征可能以组织特异性方式促成这些黏附结构的形态发生可塑性。
