Ayer-LeLievre C, Olson L, Ebendal T, Seiger A, Persson H
Department of Medical Chemistry, Karolinska Institutet, Stockholm, Sweden.
Science. 1988 Jun 3;240(4857):1339-41. doi: 10.1126/science.2897715.
In situ hybridization with complementary DNA probes for nerve growth factor (NGF) was used to identify cells containing NGF messenger RNA in rat and mouse brain. The most intense labeling occurred in hippocampus, where hybridizing neurons were found in the dentate gyrus and the pyramidal cell layer. The neuronal identity of NGF mRNA-containing cells was further assessed by a loss of NGF-hybridizing mRNA in hippocampal areas where neurons had been destroyed by kainic acid or colchicine. RNA blot analysis also revealed a considerable decrease in the level of NGF mRNA in rat dentate gyrus after a lesion was produced by colchicine. This lesion also caused a decrease in the level of Thy-1 mRNA and an increase in the level of glial fibrillary acidic protein mRNA. Neuronal death was thus associated with the disappearance of NGF mRNA. These results suggest a synthesis of NGF by neurons in the brain and imply that, in hippocampus, NGF influences NGF-sensitive neurons through neuron-to-neuron interactions.
利用神经生长因子（NGF）互补DNA探针进行原位杂交，以鉴定大鼠和小鼠脑中含有NGF信使核糖核酸（mRNA）的细胞。标记最强的区域出现在海马体，在齿状回和锥体细胞层发现了杂交神经元。通过在海马体区域中用红藻氨酸或秋水仙碱破坏神经元后，含有NGF杂交mRNA的细胞中NGF杂交mRNA的缺失，进一步评估了含NGF mRNA细胞的神经元特性。RNA印迹分析还显示，秋水仙碱造成损伤后，大鼠齿状回中NGF mRNA水平显著下降。这种损伤还导致Thy-1 mRNA水平降低，胶质纤维酸性蛋白mRNA水平升高。因此，神经元死亡与NGF mRNA的消失有关。这些结果表明，脑中的神经元可合成NGF，并且意味着在海马体中，NGF通过神经元间的相互作用影响对NGF敏感的神经元。
