Zhang Tiran, Liu Liqiang, Fan Jincai, Tian Jia, Gan Cheng, Yang Zengjie, Jiao Hu, Han Bing, Liu Zheng
Plastic Surgery Hospital, Peking Union Medical College (PUMC), CAMS, 33#, Badachu Road, Shijingshan District, Beijing, China.
Lasers Med Sci. 2017 Jul;32(5):1189-1195. doi: 10.1007/s10103-017-2224-8. Epub 2017 May 16.
This study was conducted in order to evaluate the role of low-level laser treatment (LLLT) in hair growth in C3H/HeJ mice. Healthy C57BL/6 mice were randomly divided into two groups: with and without low-level laser treatment. The skin color of each mouse was observed each day. Skin samples were collected for H&E, immunofluorescence, PCR, and western blot analysis, to observe the morphology of hair follicles and detect the expression levels of Wnt10b and β-catenin. Observation of skin color demonstrated that black pigmentation started significantly earlier in the laser group than in the control group. Hair follicle number in both groups showed no difference; however, the hair follicle length presented a significant difference. Wnt10b protein was detected on the second day in hair matrix cells in the LLLT group but not in the control group. PCR and western blot results both illustrated that expression of Wnt10b and β-catenin was significantly higher in the LLLT group than in the control group. Our study illustrated that low-level laser treatment can promote hair regrowth by inducing anagen phase of hair follicles via initiating the Wnt10b/β-catenin pathway.
本研究旨在评估低强度激光治疗（LLLT）对C3H/HeJ小鼠毛发生长的作用。将健康的C57BL/6小鼠随机分为两组：接受低强度激光治疗组和未接受低强度激光治疗组。每天观察每只小鼠的皮肤颜色。收集皮肤样本进行苏木精-伊红染色（H&E）、免疫荧光、聚合酶链反应（PCR）和蛋白质印迹分析，以观察毛囊形态并检测Wnt10b和β-连环蛋白的表达水平。皮肤颜色观察表明，激光治疗组的黑色素沉着开始时间明显早于对照组。两组的毛囊数量无差异；然而，毛囊长度存在显著差异。在低强度激光治疗组的毛母质细胞中，第二天检测到Wnt10b蛋白，而对照组未检测到。PCR和蛋白质印迹结果均表明，低强度激光治疗组中Wnt10b和β-连环蛋白的表达明显高于对照组。我们的研究表明，低强度激光治疗可通过启动Wnt10b/β-连环蛋白通路诱导毛囊进入生长期，从而促进毛发再生。
