Simi S, Colella C M, Mariani T, Piras A, Rainaldi G
Istituto di Mutagenesi e Differenziamento, C.N.R., Pisa, Italy.
Teratog Carcinog Mutagen. 1988;8(1):45-54. doi: 10.1002/tcm.1770080106.
Colcemid is known to inhibit the spindle formation and to induce polyploidy and chromosomal nondisjunction. Using a V79 Chinese hamster cell line, we have shown that colcemid is able to induce the formation of cells that are numerically diploid but whose karyotype, when analyzed with the G-banding technique, differs from that of the untreated ones. Even though these cells have a normal chromosomal constitution, they carry alterations in the chromosomal balance and, consequently, in gene dosage. This could result in an abnormal expression of cellular genes or in the expression of new or preexisting recessive mutations, even in a diploid chromosomal constitution.
已知秋水仙酰胺可抑制纺锤体形成，并诱导多倍体和染色体不分离。利用V79中国仓鼠细胞系，我们已经证明秋水仙酰胺能够诱导形成数量上为二倍体但用G带技术分析时其核型与未处理细胞不同的细胞。即使这些细胞具有正常的染色体组成，但它们的染色体平衡发生了改变，因此基因剂量也发生了改变。这可能导致细胞基因的异常表达，或者导致新的或先前存在的隐性突变的表达，即使在二倍体染色体组成中也是如此。
