Fan Haitian, Conn Adam B, Williams Preston B, Diggs Stephen, Hahm Joseph, Gamper Howard B, Hou Ya-Ming, O'Leary Seán E, Wang Yinsheng, Blaha Gregor M
Department of Biochemistry, University of California, Riverside, CA 92521, USA.
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Nucleic Acids Res. 2017 Nov 2;45(19):11043-11055. doi: 10.1093/nar/gkx719.
In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation. The interactions between RNA polymerase and ribosomes are crucial for the coordination of transcription with translation. Here, we report that RNA polymerase directly binds ribosomes and isolated large and small ribosomal subunits. RNA polymerase and ribosomes form a one-to-one complex with a micromolar dissociation constant. The formation of the complex is modulated by the conformational and functional states of RNA polymerase and the ribosome. The binding interface on the large ribosomal subunit is buried by the small subunit during protein synthesis, whereas that on the small subunit remains solvent-accessible. The RNA polymerase binding site on the ribosome includes that of the isolated small ribosomal subunit. This direct interaction between RNA polymerase and ribosomes may contribute to the coupling of transcription to translation.
在原核生物中，RNA聚合酶和核糖体可以同时结合到同一个RNA转录本上，从而导致转录和翻译的功能偶联。RNA聚合酶与核糖体之间的相互作用对于转录与翻译的协调至关重要。在此，我们报道RNA聚合酶直接结合核糖体以及分离出的核糖体大亚基和小亚基。RNA聚合酶与核糖体形成一种解离常数为微摩尔级的一对一复合物。该复合物的形成受到RNA聚合酶和核糖体的构象及功能状态的调节。在蛋白质合成过程中，核糖体大亚基上的结合界面被小亚基掩埋，而小亚基上的结合界面仍可接触溶剂。核糖体上的RNA聚合酶结合位点包括分离出的核糖体小亚基的结合位点。RNA聚合酶与核糖体之间的这种直接相互作用可能有助于转录与翻译的偶联。
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