Singh R P, Tiwari A K, Negi B S
Indian Veterinary Research Institute, Mukteswar-Kumaon, Nainital, India.
Trop Anim Health Prod. 1998 Aug;30(4):229-32. doi: 10.1023/a:1005023111485.
Laboratory diagnosis of goat poxvirus (GPV) requires suitable diagnostic reagents (sera and antigens). GPV-infected scab suspension has been used as antigen for production of hyperimmune sera (HIS) by several workers (Pandey and Singh, 1972; Tantawi et al., 1980; Sharma et al., 1988). This antiserum sometimes reacts non-specifically in routine laboratory tests such as the agar gel precipitation test (AGPT) and counter-immunoelectrophoresis (CIE) (Sharma et al., 1988). Production of specific goat pox antiserum involves its adsorption with healthy goat-skin triturate. The present work evaluated various HIS raised against different antigens of GPV in order to develop an antiserum for laboratory diagnosis of goat pox infections without any non-specificity.
