Stathmin的过表达在食管鳞状细胞癌转移中起关键作用。

Overexpression of stathmin plays a pivotal role in the metastasis of esophageal squamous cell carcinoma.

作者信息

Han Gaijing, Wu Zongyong, Zhao Nan, Zhou Lanping, Liu Fang, Niu Fangfei, Xu Yang, Zhao Xiaohang

机构信息

State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

出版信息

Oncotarget. 2017 Jun 27;8(37):61742-61760. doi: 10.18632/oncotarget.18687. eCollection 2017 Sep 22.

PURPOSE

Esophageal squamous cell carcinoma (ESCC) is a serious malignant tumor that affects human health. We analyzed the correlation between serum stathmin level and ESCC and elucidated the molecular mechanisms of stathmin's promotion of ESCC cell invasion and metastasis.

METHODS

Stathmin level in ESCC and healthy control serum were detected by enzyme-linked immunosorbent assay (ELISA), and the clinical parameters were analyzed. We established ESCC cells with stathmin overexpression or knockdown and then evaluated the effects of stathmin on invasion and metastasis in ESCC. Differentially expressed genes were analyzed by Human Transcriptome Array and confirmed by RT-PCR. The expression levels of the integrin family, focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK) were detected by immunoblotting.

RESULTS

Serum levels of stathmin were significantly higher in ESCC than in control serum and associated with lymph node metastasis, tumor stage and size. Furthermore, we found that stathmin promoted migration and invasion of ESCC cells and . In addition, we confirmed that the activation of the integrinα5β1/FAK/ERK pathway is increased in stathmin-overexpression cells and accelerates cell motility by enhancing cell adhesion ability.

CONCLUSION

Stathmin may predict a potential metastasis biomarker for ESCC.

目的

食管鳞状细胞癌（ESCC）是一种严重影响人类健康的恶性肿瘤。我们分析了血清中微管蛋白（Stathmin）水平与ESCC之间的相关性，并阐明了Stathmin促进ESCC细胞侵袭和转移的分子机制。

方法

采用酶联免疫吸附测定（ELISA）检测ESCC患者和健康对照血清中Stathmin水平，并分析临床参数。我们构建了Stathmin过表达或敲低的ESCC细胞系，然后评估Stathmin对ESCC侵袭和转移的影响。通过人类转录组芯片分析差异表达基因，并通过逆转录聚合酶链反应（RT-PCR）进行验证。通过免疫印迹法检测整合素家族、黏着斑激酶（FAK）和细胞外信号调节激酶（ERK）的表达水平。