Evans J W, Hughes J P, Neely D P, Stabenfeldt G H, Winger C M
J Reprod Fertil Suppl. 1979(27):143-50.
Jugular blood samples were obtained from 8 mares at 5- and/or 20-min intervals for 2 to 5 days during various phases of the oestrous cycle for plasma LH determination. An episodic release pattern was observed in 1 of 3 mares sampled during the ovulatory period. One mare had one secretory burst and the other mare had several periods of fluctuating plasma LH concentration. During dioestrus, episodic secretions were observed in 2 mares sampled 11 to 13 days before and, in 1 mare, 9 days after ovulation. During the 2 to 5-day period before ovulation, episodic secretion was not observed (3 mares) but plasma LH concentrations fluctuated as much as 6 ng/ml during a period of 3--4 h. Daily plasma samples were obtained form 10 mares (1--8 oestrous cycles/mare) during which 22 single, 18 double and 2 luteal-phase ovulations occurred. Dioestrous ovulations were accompanied by small increases in plasma LH (1--4 ng/ml), but many similar increases in LH were not accompanied by ovulation. No significant differences in secretory patterns were observed between single and multiple ovulations. In one mare, 4 ovulations occurred in the presence of a prolonged luteal phase; 3 were accompanied by increasing LH concentrations and the other occurred when LH was at a low concentration.
在发情周期的不同阶段，每隔5分钟和/或20分钟从8匹母马采集颈静脉血样，持续2至5天，用于测定血浆促黄体生成素（LH）。在排卵期采集血样的3匹母马中，有1匹观察到了间歇性释放模式。1匹母马出现了一次分泌高峰，另一匹母马的血浆LH浓度有几个波动期。在间情期，在排卵前11至13天采集血样的2匹母马以及排卵后9天采集血样的1匹母马中观察到了间歇性分泌。在排卵前2至5天的时间段内，未观察到间歇性分泌（3匹母马），但在3至4小时内血浆LH浓度波动高达6 ng/ml。从10匹母马（每匹母马1至8个发情周期）采集每日血浆样本，在此期间发生了22次单次排卵、18次双排卵和2次黄体期排卵。间情期排卵伴随着血浆LH的小幅升高（1至4 ng/ml），但许多类似的LH升高并未伴随排卵。单次排卵和多次排卵之间在分泌模式上未观察到显著差异。在1匹母马中，在黄体期延长的情况下发生了4次排卵；其中3次伴随着LH浓度升高，另一次发生在LH浓度较低时。
