Naus C C, Morrison J H, Bloom F E
Division of Preclinical Neuroscience and Endocrinology, Research Institute of the Scripps Clinic, La Jolla, CA 92037.
Brain Res. 1988 May 1;468(1):113-21. doi: 10.1016/0165-3806(88)90013-2.
Using a combination of in situ hybridization and immunohistochemistry, the development of somatostatin (SS)-containing neurons and fibers was examined in the rat dorsal hippocampus and dentate gyrus. The major development of this hippocampal peptidergic system occurs postnatally. At postnatal day 1 (P1), neurons containing SS mRNA are evident primarily in the stratum oriens, but also in the hilus of the dentate gyrus. Similar neurons are also immunoreactive for SS28 and SS28(1-12), suggesting a minimal lag in the transcription of SS mRNA and its translation into specific SS peptides. The number of SS neurons increases postnatally to P10, followed by a decrease in number in the adult. This transient change in the number of SS neurons coincides with dramatic changes in SS28(1-12)-immunoreactive fibers, which are initially present in the stratum lacunosum moleculare, with no significant immunoreactivity in the dentate gyrus. By P15, the molecular layer of the dentate gyrus is densely innervated, while similar immunoreactivity in the stratum lacunosum moleculare is greatly reduced. These data are consistent with a transient projection from the stratum oriens to the stratum lacunosum moleculare, which is replaced by a projection from the hilus to the molecular layer of the dentate gyrus as this structure matures.
运用原位杂交和免疫组织化学相结合的方法，研究了大鼠背侧海马和齿状回中含生长抑素（SS）的神经元和纤维的发育情况。该海马肽能系统的主要发育发生在出生后。出生后第1天（P1），含SS mRNA的神经元主要出现在海马下托，也出现在齿状回的门区。类似的神经元对SS28和SS28(1 - 12)也呈免疫反应性，这表明SS mRNA的转录及其翻译成特定的SS肽之间存在最小延迟。SS神经元的数量在出生后增加至P10，随后在成年期数量减少。SS神经元数量的这种短暂变化与SS28(1 - 12)免疫反应性纤维的显著变化相吻合，这些纤维最初出现在分子层，齿状回中无明显免疫反应性。到P15时，齿状回的分子层被密集支配，而分子层中的类似免疫反应性则大大降低。这些数据与从海马下托到分子层的短暂投射一致，随着该结构成熟，这种投射被从门区到齿状回分子层的投射所取代。
